Notch Signaling: Methods and Protocols

Notch Signaling: Methods and Protocols focuses on molecular, biochemical and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage and transcriptional targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Notch Signaling: Methods and Protocols is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor.

Table of Contents

Chapter 1 Introduction to Notch Signaling
Chapter 2 Genetic Screens to Identify New Notch Pathway Mutants in Drosophila
Chapter 3 Structure-Function Analysis of Drosophila Notch Using Genomic Rescue Transgenes
Chapter 4 Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice
Chapter 5 Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster
Chapter 6 Antibody Uptake Assay and In Vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila
Chapter 7 Tracking Trafficking of Notch and Its Ligands in Mammalian Cells
Chapter 8 Visualizing Notch Signaling In Vivo in Drosophila Tissues
Chapter 9 Monitoring Notch Activity in the Mouse
Chapter 10 Notch Signaling Assays in Drosophila Cultured Cell Lines
Chapter 11 Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays
Chapter 12 Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays
Chapter 13 Visualization of Notch Signaling Oscillation in Cells and Tissues
Chapter 14 Proteomic Analysis of the Notch Interactome
Chapter 15 Bacterial Expression and In Vitro Refolding of Limited Fragments of the Notch Receptor and Its Ligands
Chapter 16 Analyzing the Posttranslational Modification Status of Notch Using Mass Spectrometry
Chapter 17 Assay to Probe Proteolytic Processing of Notch by γ-Secretase
Chapter 18 Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift Assay
Chapter 19 Identifying Direct Notch Transcriptional Targets Using the GSI-Washout Assay
Chapter 20 Probing the Epigenetic Status at Notch Target Genes
Chapter 21 Notch-Ligand Binding Assays in Drosophila Cells
Chapter 22 Modeling Notch Signaling: A Practical Tutorial
Chapter 23 Small Molecules That Inhibit Notch Signaling
Chapter 24 Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling
Chapter 25 Application of Anti-ligand Antibodies to Inhibit Notch Signaling